Bioanalytical method for vinpocetine and apovincamine acid from human plasma by LC-MS/MS was written by Dongare, B. B.;Kashid, B. B.;Ghanvat, A. A.. And the article was included in Journal of Applicable Chemistry (Lumami, India) in 2018.HPLC of Formula: 42971-09-5 This article mentions the following:
A sensitive and reproducible liquid chromatog.-electro spray ionization-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of vinpocetine (VP) and its active metabolite apovincamine acid (AVA) in human plasma, with racem Pr vinpocetine(VP-IS) and racem Pr apovincamine acid (AVA-IS) as an internal standard (IS). The analyte was extracted with Solid Phase Extraction using ion exchange cartridges and analyzed on a Zorbax SB-CN (250 mm × 4.6 mm, 5μm) column. The mobile phase was composed of methanol 10 mM ammonium acetate with 0.1% Formic Acid (70:30). Vinpocetine, apovincamine acid and IS racem Pr vinpocetine, racem Pr apovincamine acid were ionized by pos. ion pneumatically assisted electro spray and detected in the multi-reaction monitoring (MRM) mode using LC-MS/MS (API 5500 QTrap)→ productions of m/z 351.4→280.1, m/z 323.2→279.2, m/z 365.3→294.1 and m/z 337.1→293.2 resp. The specificity, matrix effect, recovery, sensitivity, linearity, accuracy, precision, and stabilities were all validated over the concentration range of 0.5-250.0 ng mL-1 for both vinpocetine and apovincamine acid. The method developed was successfully and demonstrated for evaluation of pharmacokinetic profile of vinpocetine and apovincamine acid in human plasma. In the experiment, the researchers used many compounds, for example, (41S,13aS)-Ethyl 13a-ethyl-2,3,41,5,6,13a-hexahydro-1H-indolo[3,2,1-de]pyrido[3,2,1-ij][1,5]naphthyridine-12-carboxylate (cas: 42971-09-5HPLC of Formula: 42971-09-5).
(41S,13aS)-Ethyl 13a-ethyl-2,3,41,5,6,13a-hexahydro-1H-indolo[3,2,1-de]pyrido[3,2,1-ij][1,5]naphthyridine-12-carboxylate (cas: 42971-09-5) belongs to naphthyridine derivatives. The naphthyridines have 10 delocalized π-electrons located in five molecular orbitals, each of which is distorted by the presence of the nitrogen atoms causing an electron drift in that direction. Enoxacin, nalidixic acid, and trovafloxacin are 1,8-naphthyridine derivatives with antibacterial properties related to the fluoroquinolones.HPLC of Formula: 42971-09-5
Referemce:
1,8-Naphthyridine – Wikipedia,
1,8-Naphthyridine | C8H6N2 – PubChem