Comparison of RNA-seq and microarray platforms for splice event detection using a cross-platform algorithm was written by Romero, Juan P.;Ortiz-Estevez, Maria;Muniategui, Ander;Carrancio, Soraya;de Miguel, Fernando J.;Carazo, Fernando;Montuenga, Luis M.;Loos, Remco;Pio, Ruben;Trotter, Matthew W. B.;Rubio, Angel. And the article was included in BMC Genomics in 2018.Reference of 1009820-21-6 This article mentions the following:
RNA-seq is a reference technol. for determining alternative splicing at genome-wide level. Exon arrays remain widely used for the anal. of gene expression, but show poor validation rate with regard to splicing events. Com. arrays that include probes within exon junctions have been developed in order to overcome this problem. We compare the performance of RNA-seq (Illumina HiSeq) and junction arrays (Affymetrix Human Transcriptome array) for the anal. of transcript splicing events. Three different breast cancer cell lines were treated with CX-4945, a drug that severely affects splicing. To enable a direct comparison of the two platforms, we adapted EventPointer, an algorithm that detects and labels alternative splicing events using junction arrays, to work also on RNA-seq data.Common results and discrepancies between the technologies were validated and/or resolved by over 200 PCR experiments As might be expected, RNA-seq appears superior in cases where the technologies disagree and is able to discover novel splicing events beyond the limitations of phys. probe-sets. We observe a high degree of coherence between the two technologies, however, with correlation of EventPointer results over 0.90. Through decimation, the detection power of the junction arrays is equivalent to RNA-seq with up to 60 million reads. Our results suggest, therefore, that exon-junction arrays are a viable alternative to RNA-seq for detection of alternative splicing events when focusing on well-described transcriptional regions. In the experiment, the researchers used many compounds, for example, 5-((3-Chlorophenyl)amino)benzo[c][2,6]naphthyridine-8-carboxylic acid (cas: 1009820-21-6Reference of 1009820-21-6).
5-((3-Chlorophenyl)amino)benzo[c][2,6]naphthyridine-8-carboxylic acid (cas: 1009820-21-6) belongs to naphthyridine derivatives. Six naphthyridine isomers exist, based on the positions of the nitrogen atoms; they can be in the 1,5, 1,6, 1,7, 1,8, 2,6, or 2,7 positions. 1,6-Naphthyridine and some of its derivatives have been reported to have medicinal, electronic, and catalytic properties. But none of these investigations has yet resulted in any practical applications. Very few metal complexes of naphthyridines other than 1,8-naphthyridine have been described.Reference of 1009820-21-6
Referemce:
1,8-Naphthyridine – Wikipedia,
1,8-Naphthyridine | C8H6N2 – PubChem