Liu, Suying published the artcileSulindac derivative-induced apoptosis in a human umbilical vein endothelial cell line ECV304, COA of Formula: C20H17FO4S, the publication is Chinese Medical Journal (Beijing, China, English Edition) (2002), 115(7), 1074-1077, database is CAplus and MEDLINE.
The objective was to investigate the effects of sulindac metabolites on proliferation and apoptosis in the human umbilical vein endothelial cell line ECV304 in vitro. Methods The proliferation profile of ECV304 was determined by Me thiazolyl tetrazolium (MTT) method. Cell cycle distribution, apoptosis, and the ultrastructure of ECV304 were detected by flow cytometry (FCM) and electron microscopy, resp. Results MTT assay showed that the sulfide inhibited the proliferation of ECV304 and its effect was dose-dependent; the IC50 was 200 μmol/L. FCM showed that the sulfide changed cell cycle distribution. The cell cycle distribution was as follows: G1 phase (control group 77.74%; sulfone group 75.63%; sulfide group 46.12%); S phase (control group 13.64%; sulfone group 16.40; sulfide group 27.26%); G2-M phase (control group 8.61%; sulfone group 7.98%; sulfide group 26.62%). The apoptosis rates in the control group, sulfone group and sulfide group were 6.08, 4.81, and 51.90%, resp. Sulfide reduced the proportion of G1 phase, increased the proportion of S phase, G2-M phase and the apoptosis rate significantly. In the sulfide-treated cells, there were nuclear fragmentation and chromosomal condensation, shrinkage of the cell and loss of contact with neighboring cells. Apoptotic bodies were observed Sulfone showed no effect on cell proliferation, cell cycle distribution, or cell morphol. Conclusions Sulfide can significantly reduce the proliferation of ECV304, change the cell cycle distribution, and arrest cells in G2-M phase where apoptosis may be induced. Sulfone has no such effects on this cell line.
Chinese Medical Journal (Beijing, China, English Edition) published new progress about 59973-80-7. 59973-80-7 belongs to naphthyridine, auxiliary class Immunology/Inflammation,COX, name is Sulindac sulfone, and the molecular formula is C20H17FO4S, COA of Formula: C20H17FO4S.
Referemce:
https://en.wikipedia.org/wiki/1,8-Naphthyridine,
1,8-Naphthyridine | C8H6N2 – PubChem